BoRgl11A (Rhamnogalacturonan lyases) from Bacteroides ovatus

BoRgl11A

En-Rgl0182

(EC. 4.2.2.23)  Rhamnogalacturonan lyases

CAZy Family: PL11

 

PROPERTIES

1. ELECTROPHORETIC PURITY

-Single band on SDS-gel electrophoresis (MW ~68 kDa)

 

Fig 1. Electrophoresis analysis of BoRgl11A. M, molecular weight marker (PageRuler Prestained Protein Ladder, Thermo Scientific); lane 1, culture lysate of E. coli BL21-pET28a-borgl11a before IPTG induction; lane 2, culture lysate of E. coli BL21-pET28a-borgl11a after IPTG induction; lane 3, BoRgl11A purified from Ni sepharose fastflow column.

 

2. SPECIFIC ACTIVITY

3.9 U/mg protein (on RG-I-AT from Adenophora tetraphylla(Thunb.)Fisch.) at pH7.0 and 30^oC

One unit of RGL activity was defined as the amount of enzyme required to generate 1 mmol of 4,5-unsaturated galacturonic acid in 1 min.

3. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES

 

Table 1 Substrate specificity of BoRgl11A

 

4. PHYSICOCHEMICAL PROPERTIES

pH Optima: 9.0

pH Stability: 7.0-11.0

Temperature Optima: 35°C

Temperature Stability: < 30°C

 

5. STORAGE CONDITIONS

The enzyme should be stored at -20°C. For assay, this enzyme should be diluted in phosphate buffer (50 mM) pH 7.0. Swirl to mix the enzyme immediately prior to use.